tlr2 apc Search Results


cd282 tlr2 apc  (Miltenyi Biotec)
95
Miltenyi Biotec cd282 tlr2 apc
Cd282 Tlr2 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd282 tlr2 apc/product/Miltenyi Biotec
Average 95 stars, based on 1 article reviews
cd282 tlr2 apc - by Bioz Stars, 2026-02
95/100 stars
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unconjugated mouse anti human tlr2  (Novus Biologicals)
93
Novus Biologicals unconjugated mouse anti human tlr2
FIGURE 1. Human monocytes and macrophages express <t>TLR2</t> and TLR4. PBMC were isolated from human blood and incubated in Teflon wells with autologous serum. The monocytes differentiate into macrophages (MDM) by day 5. One (A and E)-, 3 (B and F)-, or 5 (C and G)-day-old PBMCs were harvested and incubated with either an allophycocyanin-conjugated human TLR2 mAb (or an allophycocyanin-conjugated subtype control mAb) or a PE-conjugated human TLR4 mAb (or a PE-conjugated subtype control mAb). The stained cells were analyzed using flow cytometry by gating on the monocytes or macrophages (4), and a representative experiment is shown. The number in the top right corner represents the specific MFI (TLR2 or TLR4 MFI subtype control MFI). D and H, Bar graph with cumulative data (triplicate samples in each experiment; n 5 for TLR2; n 4 for TLR4). One-way ANOVA with post-Tukey test, , p 0.005 compared with day 1 cells (SEM).
Unconjugated Mouse Anti Human Tlr2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/unconjugated mouse anti human tlr2/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
unconjugated mouse anti human tlr2 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


FIGURE 1. Human monocytes and macrophages express TLR2 and TLR4. PBMC were isolated from human blood and incubated in Teflon wells with autologous serum. The monocytes differentiate into macrophages (MDM) by day 5. One (A and E)-, 3 (B and F)-, or 5 (C and G)-day-old PBMCs were harvested and incubated with either an allophycocyanin-conjugated human TLR2 mAb (or an allophycocyanin-conjugated subtype control mAb) or a PE-conjugated human TLR4 mAb (or a PE-conjugated subtype control mAb). The stained cells were analyzed using flow cytometry by gating on the monocytes or macrophages (4), and a representative experiment is shown. The number in the top right corner represents the specific MFI (TLR2 or TLR4 MFI subtype control MFI). D and H, Bar graph with cumulative data (triplicate samples in each experiment; n 5 for TLR2; n 4 for TLR4). One-way ANOVA with post-Tukey test, , p 0.005 compared with day 1 cells (SEM).

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.

doi: 10.4049/jimmunol.180.12.7847

Figure Lengend Snippet: FIGURE 1. Human monocytes and macrophages express TLR2 and TLR4. PBMC were isolated from human blood and incubated in Teflon wells with autologous serum. The monocytes differentiate into macrophages (MDM) by day 5. One (A and E)-, 3 (B and F)-, or 5 (C and G)-day-old PBMCs were harvested and incubated with either an allophycocyanin-conjugated human TLR2 mAb (or an allophycocyanin-conjugated subtype control mAb) or a PE-conjugated human TLR4 mAb (or a PE-conjugated subtype control mAb). The stained cells were analyzed using flow cytometry by gating on the monocytes or macrophages (4), and a representative experiment is shown. The number in the top right corner represents the specific MFI (TLR2 or TLR4 MFI subtype control MFI). D and H, Bar graph with cumulative data (triplicate samples in each experiment; n 5 for TLR2; n 4 for TLR4). One-way ANOVA with post-Tukey test, , p 0.005 compared with day 1 cells (SEM).

Article Snippet: Unconjugated mouse anti-human TLR2 (clone TL2.1; Novus Biologicals), mouse anti-human TLR4 (clone HTA125; Gene Tex), mouse IgG2a (clone 20102; R&D Systems), mouse anti-human MR (clone 19.2; BD Biosciences), and mouse IgG1 (R&D Systems) were used for confocal microscopy experiments in which Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes and Invitrogen Detection Technologies) was used as a secondary Ab.

Techniques: Isolation, Incubation, Control, Staining, Cytometry

FIGURE 2. TLR2 steady-state mRNA levels decrease, while TLR4 levels vary to only a small extent, as monocytes differentiate into macrophages. One-, 3-, and 5- day-old PBMC in Teflon wells were harvested and adhered to a 12-well tissue culture plate in RPMI containing 10% autologous serum. After washing away lymphocytes, the monocytes or MDMs were lysed in TRIzol and total RNA was isolated. mRNA was converted to cDNA and real-time PCR was performed. TLR2 (A), TLR4 (B), and MR (C) amplification was normalized to the -actin housekeeping gene and the RCN number was determined. A and B, Representative experiments (mean SD, triplicate samples) and C is cumulative data (mean SEM) (n 6 (TLR2, TLR4); n 4 (MR)). The MR was used as a positive control as a macrophage marker. One-way ANOVA with post-Tukey test. , p 0.05 compared with day 1 monocytes. , p 0.005 compared with day 1 monocytes.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.

doi: 10.4049/jimmunol.180.12.7847

Figure Lengend Snippet: FIGURE 2. TLR2 steady-state mRNA levels decrease, while TLR4 levels vary to only a small extent, as monocytes differentiate into macrophages. One-, 3-, and 5- day-old PBMC in Teflon wells were harvested and adhered to a 12-well tissue culture plate in RPMI containing 10% autologous serum. After washing away lymphocytes, the monocytes or MDMs were lysed in TRIzol and total RNA was isolated. mRNA was converted to cDNA and real-time PCR was performed. TLR2 (A), TLR4 (B), and MR (C) amplification was normalized to the -actin housekeeping gene and the RCN number was determined. A and B, Representative experiments (mean SD, triplicate samples) and C is cumulative data (mean SEM) (n 6 (TLR2, TLR4); n 4 (MR)). The MR was used as a positive control as a macrophage marker. One-way ANOVA with post-Tukey test. , p 0.05 compared with day 1 monocytes. , p 0.005 compared with day 1 monocytes.

Article Snippet: Unconjugated mouse anti-human TLR2 (clone TL2.1; Novus Biologicals), mouse anti-human TLR4 (clone HTA125; Gene Tex), mouse IgG2a (clone 20102; R&D Systems), mouse anti-human MR (clone 19.2; BD Biosciences), and mouse IgG1 (R&D Systems) were used for confocal microscopy experiments in which Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes and Invitrogen Detection Technologies) was used as a secondary Ab.

Techniques: Isolation, Real-time Polymerase Chain Reaction, Positive Control, Marker

FIGURE 3. SP-A increases TLR2, but not TLR4, surface expression on MDMs. Five-day old PBMC in Teflon wells were incubated with or without SP-A (10 g/ml) for 2 h (A and B). Following the same protocol as in Fig. 1, SP-A-treated and untreated MDMs were incubated with either a human allophycocyanin-conjugated TLR2 mAb or allophycocyanin-conjugated subtype control mAb (A) or human PE-conjugated TLR4 mAb or PE-conjugated subtype control mAb (B). The stained cells were analyzed using flow cytometry by gating on the MDMs and the average of triplicate samples is shown in this experiment which is representative of n 4 (A) and n 5 (B). Five-day-old MDMs in monolayer culture on glass coverslips were incubated with or without SP-A (10 g/ml) for 2 h. After washing, cells were fixed in paraformaldehyde (no permeabilization) and stained with mouse anti-human TLR2 mAb, mouse anti-human TLR4 mAb, mouse anti-human MR mAb, or subtype control mAb followed by a secondary Alexa Fluor 488-conjugated anti-mouse Ab. Glass coverslips were mounted and visualized by confocal microscopy. A representative experiment is shown in C (n 3 (TLR2); n 2 (TLR4, MR)). Cumulative data are shown in D. A Student t test was performed. , p 0.05 relative to TLR2 expression on untreated MDMs. The MR was used as a positive control.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.

doi: 10.4049/jimmunol.180.12.7847

Figure Lengend Snippet: FIGURE 3. SP-A increases TLR2, but not TLR4, surface expression on MDMs. Five-day old PBMC in Teflon wells were incubated with or without SP-A (10 g/ml) for 2 h (A and B). Following the same protocol as in Fig. 1, SP-A-treated and untreated MDMs were incubated with either a human allophycocyanin-conjugated TLR2 mAb or allophycocyanin-conjugated subtype control mAb (A) or human PE-conjugated TLR4 mAb or PE-conjugated subtype control mAb (B). The stained cells were analyzed using flow cytometry by gating on the MDMs and the average of triplicate samples is shown in this experiment which is representative of n 4 (A) and n 5 (B). Five-day-old MDMs in monolayer culture on glass coverslips were incubated with or without SP-A (10 g/ml) for 2 h. After washing, cells were fixed in paraformaldehyde (no permeabilization) and stained with mouse anti-human TLR2 mAb, mouse anti-human TLR4 mAb, mouse anti-human MR mAb, or subtype control mAb followed by a secondary Alexa Fluor 488-conjugated anti-mouse Ab. Glass coverslips were mounted and visualized by confocal microscopy. A representative experiment is shown in C (n 3 (TLR2); n 2 (TLR4, MR)). Cumulative data are shown in D. A Student t test was performed. , p 0.05 relative to TLR2 expression on untreated MDMs. The MR was used as a positive control.

Article Snippet: Unconjugated mouse anti-human TLR2 (clone TL2.1; Novus Biologicals), mouse anti-human TLR4 (clone HTA125; Gene Tex), mouse IgG2a (clone 20102; R&D Systems), mouse anti-human MR (clone 19.2; BD Biosciences), and mouse IgG1 (R&D Systems) were used for confocal microscopy experiments in which Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes and Invitrogen Detection Technologies) was used as a secondary Ab.

Techniques: Expressing, Incubation, Control, Staining, Cytometry, Confocal Microscopy, Positive Control

FIGURE 4. SP-A regulates steady-state mRNA ex- pression of TLR2, but not TLR4, during monocyte dif- ferentiation into macrophages. SP-A (10 g/ml) was added to 1-, 3-, and 5-day-old PBMC in selected Teflon wells for 1, 2, 6, or 20 h. PBMC were harvested and monocytes/macrophages adhered to a 12-well tissue culture plate in autologous serum. After washing away lymphocytes, the monocytes or MDMs were lysed in TRIzol and total RNA was isolated. mRNA was con- verted to cDNA and real-time PCR was performed. TLR2 and TLR4 mRNA amplification was normalized to the -actin housekeeping gene. The fold change was determined by comparing SP-A-treated samples to sam- ples with no SP-A. Cumulative data are shown (SEM) (TLR2: n 6 (day 5, 20 h); n 5 (day 5, 2 h); n 4 (day 1, 1, 2, 6, and 20 h; day 3, 1 and 2 h; day 5 1 and 6 h); n 2 (day 3, 6 and 20 h)) (TLR4: n 6 (day 1, 2 h); n 5 (day 1, 1 and 20 h); n 4 (day 1, 6 h); n 3 (day 3, 1 and 2 h; day 5, 2 and 20 h); n 2 (day 3, 6 h; day 5, 6 h); n 1 (day 3, 20 h; day 5, 20 h)). Student t test was performed. , p 0.05 compared with day 1 monocytes.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.

doi: 10.4049/jimmunol.180.12.7847

Figure Lengend Snippet: FIGURE 4. SP-A regulates steady-state mRNA ex- pression of TLR2, but not TLR4, during monocyte dif- ferentiation into macrophages. SP-A (10 g/ml) was added to 1-, 3-, and 5-day-old PBMC in selected Teflon wells for 1, 2, 6, or 20 h. PBMC were harvested and monocytes/macrophages adhered to a 12-well tissue culture plate in autologous serum. After washing away lymphocytes, the monocytes or MDMs were lysed in TRIzol and total RNA was isolated. mRNA was con- verted to cDNA and real-time PCR was performed. TLR2 and TLR4 mRNA amplification was normalized to the -actin housekeeping gene. The fold change was determined by comparing SP-A-treated samples to sam- ples with no SP-A. Cumulative data are shown (SEM) (TLR2: n 6 (day 5, 20 h); n 5 (day 5, 2 h); n 4 (day 1, 1, 2, 6, and 20 h; day 3, 1 and 2 h; day 5 1 and 6 h); n 2 (day 3, 6 and 20 h)) (TLR4: n 6 (day 1, 2 h); n 5 (day 1, 1 and 20 h); n 4 (day 1, 6 h); n 3 (day 3, 1 and 2 h; day 5, 2 and 20 h); n 2 (day 3, 6 h; day 5, 6 h); n 1 (day 3, 20 h; day 5, 20 h)). Student t test was performed. , p 0.05 compared with day 1 monocytes.

Article Snippet: Unconjugated mouse anti-human TLR2 (clone TL2.1; Novus Biologicals), mouse anti-human TLR4 (clone HTA125; Gene Tex), mouse IgG2a (clone 20102; R&D Systems), mouse anti-human MR (clone 19.2; BD Biosciences), and mouse IgG1 (R&D Systems) were used for confocal microscopy experiments in which Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes and Invitrogen Detection Technologies) was used as a secondary Ab.

Techniques: Isolation, Real-time Polymerase Chain Reaction

FIGURE 8. SP-A is a key regulator of TLR expression and signaling in macrophages. SP-A binds to its receptor(s) leading to increased expression of TLR2 but not TLR4 (this report), the MR () (4) and SR-A () (5). Simultaneously, SP-A regulates TLR activity in response to agonists by decreasing the phosphorylation of key TLR signaling proteins, including Akt and MAPKs. Finally, SP-A decreases the phosphorylation of IB and nuclear translocation of p65 which results in diminished proinflammatory cytokine production.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Pulmonary surfactant protein A regulates TLR expression and activity in human macrophages.

doi: 10.4049/jimmunol.180.12.7847

Figure Lengend Snippet: FIGURE 8. SP-A is a key regulator of TLR expression and signaling in macrophages. SP-A binds to its receptor(s) leading to increased expression of TLR2 but not TLR4 (this report), the MR () (4) and SR-A () (5). Simultaneously, SP-A regulates TLR activity in response to agonists by decreasing the phosphorylation of key TLR signaling proteins, including Akt and MAPKs. Finally, SP-A decreases the phosphorylation of IB and nuclear translocation of p65 which results in diminished proinflammatory cytokine production.

Article Snippet: Unconjugated mouse anti-human TLR2 (clone TL2.1; Novus Biologicals), mouse anti-human TLR4 (clone HTA125; Gene Tex), mouse IgG2a (clone 20102; R&D Systems), mouse anti-human MR (clone 19.2; BD Biosciences), and mouse IgG1 (R&D Systems) were used for confocal microscopy experiments in which Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes and Invitrogen Detection Technologies) was used as a secondary Ab.

Techniques: Expressing, Activity Assay, Phospho-proteomics, Translocation Assay